尽管半个世纪前对利什曼原虫的血清学分类法已作过一些初步尝试,但多年来进展缓慢。起初,许多标准的血清学方法,如凝集反应,补体结合试验对利什曼属的特异性不强。以后发现,广泛用于利什曼病血清流行病学调查的间接荧光抗体法同样无种的特异性,所以未能用于虫种的鉴定。以上方法的特异性较差,可能是由于利什曼抗原成分的复杂性,而在许多情况下,在制备抗原时虫体破坏而难以发现种特异性的成分。Adler成功地建立了一种鉴定利什曼原虫的特异性高的方法。此法的基础是利什曼原虫在含有同 Although some initial attempts have been made to identify the serological classification of Leishmania half a century ago, its progress has been slow for many years. Initially, many standard serological methods, such as agglutination, complement fixation tests are not specific to Leishmania. It was later found that the indirect fluorescent antibody method widely used for the serological epidemiological investigation of leishmaniasis was also seed-free and therefore not used for the identification of insect species. The poor specificity of the above method may be due to the complexity of the components of Leishman’s antigens, and in many cases, it is difficult to find species-specific components when the antigen is prepared. Adler has successfully established a highly specific method of identifying Leishmania. The basis of this method is that Leishmania contains the same