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目的：探讨 miR-29c 在蛛网膜下腔出血（SAH）后柔脑膜纤维化过程中的作用。方法选用健康雄性 SD 大鼠26只按随机数字表法分为假手术组（6只）、空白慢病毒组（8只）、LV-rno-miRNA-29c 组（12只）。侧脑室分别注入5μL 生理盐水、空白慢病毒、LV-rno-miRNA-29c，注射1周后，通过枕大池二次注血法建立蛛网膜下腔出血模型。造模21 d 后处死大鼠，采用 Realtime PCR 法检测柔脑膜内转化生长因子β1（TGF-β1）及 miR-29c 表达量，酶联免疫吸附法（ELISA）检测脑脊液（CSF）中Ⅰ型胶原前端肽（PICP）含量，柔脑膜 Masson 染色观察柔脑膜胶原纤维。结果SAH 后柔脑膜中 TGF-β1表达升高，miR-29c 表达下调；上调 miR-29c 能显著抑制脑脊液中 PICP 及柔脑膜中胶原表达。结论维持 miR-29c 高表达可抑制 SAH 后柔脑膜胶原合成，从而治疗柔脑膜纤维化。“,”Objective To explore the role of miR-29c in rat leptomeningeal fibrosis after subarachnoid hemorrhage (SAH).Methods A total of 26 healthy Sprague-Dawley (SD)rats were randomly divided into sham group (n =6), empty lentiviral group (n =8),and LV-rno-miRNA-29c group (n =12).5 μL saline,empty lentivirus and LV-rno-miRNA-29c were respectively injected into the lateral cerebral ventricle of rats in each group.The SAH models were induced by injecting autologous blood twice into the cisterna magna,and 21 days later the rats were killed.Realtime PCR was used to detect the expressions of TGF-β1 and miR-29c.Masson staining was performed to observe collagen fiber.ELISA was adopted to measure the level of PICP in CSF.Results Increased TGF-β1 and decreased miR-29c were detected.The upregulation of miR-29c could significantly reduce the expressions of PICP in CSF and collagen in leptomeninge.Conclusion High expression of miR-29c may have potential therapeutic effects for leptomeningeal fi-brosis by inhibiting collagen synthesis after SAH.