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利用化学发光分析技术建立一种新的一氧化氮合成酶（ＮＯＳ）活性测定方法。Ｌ－精氨酸在多种辅助因子的参与下，经ＮＯＳ的催化生成一氧化氮（ＮＯ）。ＮＯ与过氧化氢反应生成过氧亚硝酸，后者能与鲁米诺产生强的发光反应，当ＮＯ浓度为０．１ｐｍｏｌ／Ｌ～１ｎｍｏｌ／Ｌ时，发光强度与ＮＯ浓度成正比；通过测量发光强度来确定ＮＯＳ的活性。用该方法重复测量１０只大鼠脑组织中的ＮＯＳ活性，其测定值为：（２８．３±６．９）ｐｍｏｌＮＯ／Ｌ／ｍｇ蛋白／ｍｉｎ；稳定性较好。此方法避免了同位素方法的繁琐及放射性污染，是一种简便易行的ＮＯＳ活性测定方法
A new method for the determination of nitric oxide synthase (NOS) activity was established by chemiluminescence analysis. L-Arginine catalyzes the production of nitric oxide (NO) by NOS with the help of various cofactors. NO reacted with hydrogen peroxide to produce peroxynitrite, which could produce strong luminescence reaction with luminol. When the concentration of NO was 0.1 pmol / L ~ 1 nmol / L, the luminescence intensity was proportional to NO concentration. By measuring Luminous intensity to determine NOS activity. The method was used to measure the NOS activity of 10 rat brain tissue repeatedly, and the measured value was (28.3 ± 6.9) pmolNO / L / mg protein / min; the stability was good. This method avoids the cumbersome and radioactive contamination of the isotope method, is a simple and easy method for the determination of NOS activity