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[目的]比较5种化学试剂诱导黄瓜对霜霉病的抗病性。[方法]待黄瓜幼苗长到4叶期时采用水杨酸、草酸、壳聚糖、硝酸钙和磷酸氢二钠诱导处理第2片真叶,7 d后测定第3片真叶防御酶系活性;同时诱导处理后进行接种试验,统计发病情况。[结果]5种化学试剂处理在不同程度上均促进黄瓜叶片POD、SOD、PAL和PPO酶活性的升高以及叶片的超微弱发光,降低了黄瓜叶片病情指数。[结论]5种试剂处理均诱导叶片产生系统抗性;诱导处理中草酸诱导抗病性的效果最为显著,其余依次是水杨酸、壳聚糖、磷酸氢二钠和硝酸钙。
[Objective] The research aimed to compare the resistance of cucumber to downy mildew induced by five chemicals. [Method] When the 4th leaf stage of cucumber seedlings was prolonged, the second true leaf was induced with salicylic acid, oxalic acid, chitosan, calcium nitrate and disodium hydrogen phosphate. The third true leaf defensive enzyme Activity; at the same time induction treatment after vaccination test statistics incidence. [Result] The five kinds of chemical agents all improved the activities of POD, SOD, PAL and PPO and the ultraweak luminescence of cucumber leaves to varying degrees, which reduced the disease index of cucumber leaves. [Conclusion] The five kinds of chemical treatments all induced systemic resistance in leaves. The most significant effect induced by oxalic acid was induced by the induction treatment, followed by salicylic acid, chitosan, disodium hydrogen phosphate and calcium nitrate.