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目的：研究脑缺血后一氧化氮合酶（ＮｉｔｒｉｃＯｘｉｄｅＳｙｎｔｈａｓｅ，ＮＯＳ）表达与细胞凋亡的关系。方法：采用大鼠右侧大脑中动脉闭塞（Ｍｉｄｄｌｅｃｅｒｅｂｒａｌａｒｔｅｒｙｏｃｃｌｕｓｉｏｎ，ＭＣＡＯ）模型，观察脑梗塞体积、ＮＯＳ表达、细胞凋亡及ＮＯＳ抑制剂硝基－Ｌ－精氨酸（Ｌ－ＮＡ）、ＮＯ供体硝普钠（ＳＮＰ）对上述结果的影响。结果：ＮＯＳ表达与缺血再灌流（ＩＲ）早期细胞凋亡有关，ＳＮＰ增加ＩＲ早期细胞凋亡，Ｌ－ＮＡ能够抑制ＩＲ早期的细胞凋亡，但使脑梗塞体积增大。结论：ＩＲ早期ＮＯＳ表达对细胞凋亡有促进作用。
Objective: To study the relationship between the expression of nitric oxide synthase (NOS) and apoptosis after cerebral ischemia. Methods: Middle cerebral artery occlusion (MCAO) model was used to observe the changes of infarct volume, NOS expression, apoptosis and NOS inhibitor N-L-arginine (L-NA) Effect of sodium nitroprusside (SNP) on these results. Results: The expression of NOS was related to the early apoptosis of ischemia / reperfusion (IR) rats. SNP increased the apoptosis of IR in early stage. L-NA could inhibit the apoptosis of IR in early stage, but increased the volume of cerebral infarction. Conclusion: NOS expression in early stage of IR can promote apoptosis.