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目的:探讨液基细胞制片(TCT)免疫细胞化学在胸腹水转移性腺癌诊断中的应用价值。方法:应用一组单克隆抗体(mAb),采用TCT免疫细胞化学染色法检测117例胸腹水细胞标本,其中腺癌102例、反应性间皮细胞增生15例,观察细胞抗原表达。结果:102例腺癌中MOC31、CEA、EMA、E-Cadherin、TTF-1和vim阳性表达率分别为100%、69%、100%、100%、30%和0.98%,des和calretinin全部呈阴性表达。在15例反应性增生间皮细胞中calretinin、vim、des、CEA和EMA的阳性表达率分别为100%、100%、60%、6%和6%,MOC31、E-Cadherin和TTF-1全部呈阴性表达。MOC31、CEA、EMA和E-Cadherin在腺癌细胞中表达特异性为100%、94%、94%和100%,敏感性100%、69%、100%和100%。des、calretinin和vim在反应性增生间皮细胞中表达特异性100%、100%和99.02%,敏感性为60%、100%和100%。结论:应用TCT免疫细胞化学方法,选择性的联合使用一组抗体(MOC31、E-Cadherin、EMA、CEA、calretinin、vim、des、TTF-1),有助于胸腹水转移性腺癌细胞与反应性间皮细胞的鉴别诊断。
Objective: To investigate the value of liquid-based cytokine (TCT) immunocytochemistry in the diagnosis of metastatic adenocarcinoma of the ascites and pleural effusion. Methods: A panel of monoclonal antibodies (mAbs) was used in this study. 117 samples of pleural and peritoneal effusion were detected by TCT immunocytochemistry. Among them, 102 cases of adenocarcinoma and 15 cases of reactive mesothelial cells were observed for antigen expression. Results: The positive rates of MOC31, CEA, EMA, E-Cadherin, TTF-1 and vim in 102 adenocarcinomas were 100%, 69%, 100%, 100%, 30% and 0.98%, respectively. Negative expression. The positive rates of calretinin, vim, des, CEA and EMA were 100%, 100%, 60%, 6% and 6% in all 15 cases of reactive hyperplasia mesothelioma and all of MOC31, E-Cadherin and TTF-1 Negative expression. MOC31, CEA, EMA and E-Cadherin expressed 100%, 94%, 94% and 100% specificity in adenocarcinoma cells with 100%, 69%, 100% and 100% sensitivity. Des, calretinin, and vim expressed specificities of 100%, 100% and 99.02% in reactive proliferative mesothelial cells with sensitivities of 60%, 100% and 100%. Conclusion: TCT immunocytochemistry can be used to selectively combine a group of antibodies (MOC31, E-Cadherin, EMA, CEA, calretinin, vim, des and TTF-1) Differential diagnosis of interstitial cells.