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目的:分析宫颈癌组织中血管内皮生长因子(VEGF)的表达、微血管密度(MVD)和HPV抗原的表达的相关性。方法:收集30例宫颈癌患者的肿瘤组织标本,其中17例鳞癌患者、11例腺癌患者、2例鳞腺癌患者,其中11例为低分化,19例为高分化。采用免疫组织化学的方法对组织中的VEGF、MVD、CD34的表达进行检测。分析蛋白的表达水平与肿瘤类型、分级、HPV抗原的关系。结果:VEGF在肿瘤组织中的表达率为100%,癌症分化程度和VEGF表达以及MVD有显著的相关性(P<0.05)。组织中MVD值平均为50.4±20.0。MVD和VEGF的表达有显著的统计学相关性(P<0.05)。HPV抗原的表达率为93.3%,HPV抗原与肿瘤类型以及分级之间无统计学相关性。HPV抗原与MVD以及VEGF的表达有显著相关性。多因素统计分析表明MVD是肿瘤分级的独立预测因素。结论:VEGF在所有肿瘤组织中都有表达,VEGF的表达和MVD有显著的相关性。HPV抗原的表达同MVD和VEGF的表达显著相关,HPV的感染增强了宫颈癌组织中的血管生成。MVD可以作为宫颈癌分级的独立预测因子,代表了肿瘤的分化程度。
Objective: To analyze the relationship between the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) and HPV antigen in cervical cancer. Methods: Tumor samples from 30 patients with cervical cancer were collected, of which 17 patients were squamous cell carcinoma, 11 patients with adenocarcinoma and 2 patients with adenocarcinoma. Among them, 11 were poorly differentiated and 19 highly differentiated. Immunohistochemistry was used to detect the expression of VEGF, MVD and CD34 in the tissues. The relationship between protein expression level and tumor type, grade and HPV antigen was analyzed. Results: The expression of VEGF in tumor tissue was 100%. There was a significant correlation between the differentiation degree of cancer and VEGF expression and MVD (P <0.05). Tissue MVD values averaged 50.4 ± 20.0. The expression of MVD and VEGF was statistically significant (P <0.05). HPV antigen expression rate was 93.3%, HPV antigen and tumor type and grade no statistically significant correlation. There was a significant correlation between HPV antigen and the expression of MVD and VEGF. Multivariate statistical analysis showed that MVD was an independent predictor of tumor grade. Conclusion: VEGF is expressed in all tumor tissues, and the expression of VEGF is significantly correlated with MVD. The expression of HPV antigens is significantly correlated with the expression of MVD and VEGF, and HPV infection enhances angiogenesis in cervical cancer tissues. MVD can be used as an independent predictor of cervical cancer classification, representing the degree of tumor differentiation.