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目的应用脂多糖(LPS)刺激人脐静脉内皮细胞(HUVECs)后,检测细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和尿激酶型纤溶酶原激活物及其受体(uPA/uPAR)的mRNA和蛋白含量的变化及辛伐他汀对其表达的影响。方法实验分为12h组和24h组,各组又分为空白对照组、脂多糖(100ng/mL)组、辛伐他汀(5μg/mL)组、辛伐他汀(5μg/mL)和脂多糖(100ng/mL)共作用组,用实时荧光定量PCR和ELISA方法测定12h组、24h组TNF-α、IL-6及uPA/uPAR的mRNA和蛋白表达的变化。结果与对照组相比,脂多糖组TNF-α、IL-6及uPA/uPAR的mRNA表达和上清液中IL-6蛋白的表达均明显增加(P均<0.05);与脂多糖组相比,辛伐他汀和脂多糖共作用组TNF-α、IL-6及uPA/uPAR的mRNA表达和上清液中IL-6蛋白的表达均明显降低(P均<0.05)。结论辛伐他汀可抑制HUVECs分泌的细胞因子TNF-α、IL-6及uPA/uPAR的表达。
Objective To investigate the effects of lipopolysaccharide (LPS) on the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and urokinase plasminogen in human umbilical vein endothelial cells (HUVECs) Activator and its receptor (uPA / uPAR) mRNA and protein content changes and simvastatin on its expression. Methods The experiment was divided into 12h group and 24h group. Each group was divided into blank control group, lipopolysaccharide group (100ng / mL), simvastatin group (5μg / mL), simvastatin (5μg / mL) and lipopolysaccharide 100ng / mL). The mRNA and protein expressions of TNF-α, IL-6 and uPA / uPAR in 12h and 24h groups were determined by real-time fluorescence quantitative PCR and ELISA. Results Compared with the control group, the mRNA expressions of TNF-α, IL-6 and uPA / uPAR in the lipopolysaccharide group and the levels of IL-6 protein in the supernatant were significantly increased (all P <0.05) The mRNA expression of TNF-α, IL-6 and uPA / uPAR and the expression of IL-6 in supernatant were significantly lower than those in simvastatin and lipopolysaccharide groups (all P <0.05). Conclusion Simvastatin can inhibit the secretion of cytokines TNF-α, IL-6 and uPA / uPAR in HUVECs.