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目的 检测 PTEN蛋白在人脑胶质瘤细胞中的表达 ,探讨 PTEN表达与胶质瘤病理分级和预后的关系 .方法 应用免疫组化方法 ,检测 6 3例人脑胶质瘤标本中的 PTEN表达 ,分析其与胶质瘤细胞恶性程度与预后的关系 .结果 PTEN阳性染色颗粒定位于细胞质中 ,在 6 3例人脑胶质瘤标本中 ,有 38例 (6 0 % ) PTEN呈阳性表达 ;低分化组的阳性表达率 ( 和 级为 37% ,11/ 30 )明显低于高分化组的阳性表达率 ( 和 级为 82 % ,2 7/ 33) ,两者之间差异显著 (P<0 .0 1) . PTEN表达阳性与阴性患者在 1或 3 a的存活率比较中 ,在 和 级胶质瘤患者中无显著性差异 (P>0 .0 5 ) ;在 级和 级患者中 ,3a的存活率比较差异显著 ,PTEN表达阳性者较阴性者的存活率高 (P<0 .0 5 ) ;PTEN阳性表达的患者中 ,高分化组 ( 和 级 )和低分化组 ( 和 级 )之间在 1或3a存活率比较差异显著 (1a存活率比较 ,P<0 .0 5 ;3a存活率比较 ,P<0 .0 5 ) .结论 PTEN基因突变或缺失在人脑胶质瘤的发生发展中起重要作用 ,与肿瘤恶性分化程度密切相关 ,也与患者预后相关 ,其表达缺失严重影响着肿瘤患者的预后
Objective To detect the expression of PTEN protein in human glioma cells and to explore the relationship between the expression of PTEN and the pathological grading and prognosis of gliomas.Methods Immunohistochemistry was used to detect the expression of PTEN in 63 human gliomas , And analyzed their relationship with the malignant degree and prognosis of glioma cells.Results PTEN positive staining particles localized in the cytoplasm, and of the 63 samples of human gliomas, 38 (60%) had positive expression of PTEN. The positive expression rate (grade 37%, 11/30) in poorly differentiated group was significantly lower than that in poorly differentiated group (grade 82%, 27/33) (P < There was no significant difference in glioma patients between PTEN positive and negative patients at 1 or 3 years (P> 0.05). In grade and grade patients , The survival rate of 3a was significantly different, and the positive rate of PTEN expression was higher than that of negative ones (P <0.05). Among the PTEN positive patients, the high differentiated group (grade) and poorly differentiated group ) In 1 or 3a survival rate was significantly different (1a survival rate, P <0. 05; 3a survival rate comparison, P <0 .0 5) .Conclusion The mutation or deletion of PTEN gene plays an important role in the occurrence and development of human glioma, which is closely related to the degree of malignant differentiation of tumor and also related to the prognosis of patients. The loss of PTEN gene affects the prognosis of tumor patients