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目的 研究重度子(癎)前期(sPE)患者的胎盘组织中Rho亚家族蛋白A(RhoA)、Rho亚家族蛋白B(RhoB)/相关卷曲螺旋形成蛋白激酶1(R0ck1)、相关卷曲螺旋形成蛋白激酶2(Rock2)蛋白分子的表达,探讨其与重度子(癎)前期发病的机制.方法 采用免疫组织化学法检测重度子(癎)前期组及正常晚期妊娠组胎盘组织中的RhoA、RhoB/Rock1、Rock2蛋白分子的表达定位及差异.结果 RhoA蛋白与RhoB蛋白及Rock1蛋白与Rock2蛋白在正常晚期妊娠组和重度子(癎)前期组胎盘滋养细胞、内皮细胞及一些间质细胞的胞浆中均有表达,以合体滋养细胞胞浆中表达为主,而Rock1蛋白与Rock2蛋白胞核中少量表达.RhoA、RhoB、Rock1、Rock2蛋白在重度子(癎)前期组的表达显著高于正常晚期妊娠组(P＜0.05).结论 RhoA、RhoB蛋白和其下游靶分子Rock1、Rock2组成的信号通路,在子(癎)前期的胎盘组织中呈高表达,可能参与了子(癎)前期的胎盘的滋养细胞侵袭障碍、胎盘的滋养细胞的凋亡、缺血及缺氧、血管的部分内皮细胞损伤及动脉血管痉挛的病理过程,提示其蛋白分子在重度子(癎)前期发病中起着重要作用.“,”Objective To investigate expressions of Ras homologue protein family A (RhoA)/RhoB and Rho-associated coiled coil Rho forming protein Kinase 1 (Rock1)/Rock2 in placenta tissues of severe preeclampsia (sPE) to clarify the molecular mechanisms of sPE.Methods The locations and expressions of RhoA/RhoB and Rock1/Rock2 between two groups were detected with immunohistochemistry.Results RhoA,RhoB,Rock1,and Rock2 were mainly distributed in cytoplasms of syncytiotrophoblasts,cytotrophoblast,endothelial cells,and endometrial stromal cells.Rock1 and Rock2 were less expressed in nucleus.The expression levels of RhoA,RhoB,Rock1,and Rock2 in sPE group were significantly higher than control group (P ＜ 0.05).Conclusions The signal pathway that consists of upstream RhoA/RhoB and downstream Rock1/Rock2 is up-regulated in sPE.It demonstrates that signal molecules including RhoA,RhoB,Rockl,and Rock2 may involve in the sPE pathogenesis through affecting shallow placenta implantation in preeclampsia,ischemia,hypoxia and apoptosis of trophoblasts,and injury of vascular endothelial cells and artery vasospasm.