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16S rRNA 序列测定是细菌分类学的基本方法之一,其序列差异是确定细菌属间及属上系统发育关系的主要标准。本文根据国际系统细菌学委员会根瘤菌分委员会的建议,采用聚合酶链反应扩增了华癸根瘤菌(Rhizobium huakuii)模式菌株103的16S rRNA 基因中约260个碱基的一个片段,并对其进行了序列测定。所得序列与其他已知根瘤菌种、属的相应片段进行了比较。结果表明,华癸根瘤菌与豌豆根瘤菌和苜蓿根瘤菌两种及与其他属的碱基差异在6.9%～14.1%(17～35个碱基),相当于根瘤菌科内已知属间的差异。结合表型特征,我们认为该种可能代表了根瘤科的一个新属。
16S rRNA sequence determination is one of the basic methods of bacterial taxonomy, and its sequence differences are the main criteria to determine the phylogenetic relationships among bacteria and their genera. In this paper, a fragment of about 260 bases in the 16S rRNA gene of Rhizobium huakuii model strain 103 was amplified by polymerase chain reaction (PCR) based on the recommendations of the International Society for Bacteriological Commission’s Rhizobium Sub-Committee. Sequencing was performed. The obtained sequences were compared with those of other known rhizobia species and genera. The results showed that there were two nucleotide differences between Rhizobium japonicum and Rhizobium pea and Rhizobium meliloti and their bases with other genera ranging from 6.9% to 14.1% (17-35 bases) The difference. Combined with the phenotypic characteristics, we believe that this species may represent a new genus of nodulation.