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据《ScientiaHorticulturae》的一篇研究报道(2013.09.005),来自日本三重大学的研究人员研究了铜和锌离子对日本梨花柱核糖核酸酶(RNase)抑制和着果的诱导作用。研究人员在研究日本梨花柱核糖核酸酶活性的抑制作用时,发现CuSO4和ZnSO4效果显著,而且抑制作用具有剂量依赖性。因为MgSO4和CaCl2不表现任何抑制作用,所以活性离子是Cu2+和Zn2+。将蛋白和CuSO4混合物柱层析装载在交联葡聚糖G-10时,Cu2+已经完全从花柱蛋白中分离出来,阳离子可能与蛋白联系不紧密。1 mM铜离子强
According to a study by Scientia Horticulturae (2013.09.005), a researcher from Mie University in Japan studied the induction of copper and zinc ions on RNase inhibition and fruit production in Japan. Researchers studying the inhibition of ribonuclear enzyme activity in pear, found CuSO4 and ZnSO4 significant effect, and inhibition in a dose-dependent manner. Because MgSO4 and CaCl2 do not show any inhibitory effect, the active ions are Cu2 + and Zn2 +. When column chromatography of protein and CuSO4 mixture on Sephadex G-10, Cu2 + has been completely separated from the motif protein, and the cations may not be closely related to the protein. 1 mM strong copper ion