目的对甘肃省流行的艾滋病病毒(HIV)开展分子流行病学调查。方法用套式聚合酶链反应对23份甘肃省HIV-1感染者外周血淋巴细胞中前病毒脱氧核糖核酸(DNA)的膜蛋白基因进行扩增,并对C2-V3及其邻区300个核苷酸序列进行测定和分析。结果甘肃省流行的HIV-1分属B’亚型(6例)、C亚型(1例)、CRF-BC亚型(16例)。其中CRF-BC亚型(70%)为主要流行毒株。结论甘肃省HIV-1流行亚型以重组毒株CRF-BC为主,在吸毒人群中广为流行;加强对高危人群监控,是遏制艾滋病在甘肃省蔓延的重中之重。 Objective To investigate the molecular epidemiology of HIV in Gansu Province. Methods 23 cases of HIV-1 infected peripheral blood lymphocyte DNA of 23 cases of Gansu province were amplified by polymerase chain reaction (PCR) The nucleotide sequence was determined and analyzed. Results The prevalence of HIV-1 in Gansu Province belonged to B ’subtype (6 cases), C subtype (1 case) and CRF-BC subtype (16 cases). Which CRF-BC subtype (70%) as the main epidemic strains. Conclusion The majority of HIV-1 subtypes in Gansu province are mainly CRF-BC, and are widely used among drug users. To strengthen the monitoring of high-risk groups is the most important factor in curbing the spread of AIDS in Gansu Province.