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目的:研究胃癌组织中人类乳头瘤病毒16型(HPV16)感染与幽门螺杆菌感染之间的相关性。方法:运用原位PCR和免疫组化技术分别检测陕西省地区胃癌组织中HPV16癌基因E6和幽门螺杆菌(Hp)。结果:在40例胃癌组织(GC)中HPV16E6的阳性率为27.5%(11/40)而在40例癌旁正常组织(GANM)中未检测到;GC组中HPV16E6阳性率明显高于GANM组(P=0.0004);贲门癌中HPV16的感染率明显高于非贲门癌(P=0.0136);胃癌中HPV16与幽门螺杆菌感染无明显相关性(P=0.0829)。HPV16与胃癌患者的性别、年龄、肿瘤浸润、淋巴结转移以及病理分级均无明显相关性(P>0.05)。结论:本研究结果提示,在胃癌的发生过程中,HPV16可能不依赖或者不与幽门螺杆菌协作而发挥作用。
Objective: To study the correlation between HPV16 infection and Helicobacter pylori infection in gastric cancer. Methods: In situ PCR and immunohistochemistry were used to detect HPV16 oncogene E6 and Helicobacter pylori (Hp) in gastric cancer tissues of Shaanxi province. Results: The positive rate of HPV16E6 in 40 cases of GC was 27.5% (11/40), but not in 40 cases of adjacent normal tissues (GANM). The positive rate of HPV16E6 in GC group was significantly higher than that in GANM group (P = 0.0004). The infection rate of HPV16 in cardia cancer was significantly higher than that in non-cardia cancer (P = 0.0136). There was no significant correlation between HPV16 and Helicobacter pylori infection in gastric cancer (P = 0.0829). There was no significant correlation between HPV16 and gender, age, tumor invasion, lymph node metastasis and pathological grade of gastric cancer (P> 0.05). Conclusions: The results of this study suggest that HPV16 may play a role, independent of or in cooperation with H. pylori, during the development of gastric cancer.