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目的研究S-8型大孔树脂精制泽兰总黄酮(酚酸)的工艺条件。方法以总黄酮(酚酸)、咖啡酸含量结合指纹图谱主要特征峰面积保留率为考察指标;采用紫外-可见分光光度法测定总黄酮(酚酸)含量,HPLC测定咖啡酸含量并分析指纹图谱主要特征峰面积。结果 S-8型大孔吸附树脂纯化泽兰总黄酮(酚酸)的最佳工艺条件为:上样液浓度为含生药量0.1g·mL-1,上样量为每10g干树脂上样7g生药,吸附速率为1mL·min-1,水洗脱量为60mL,洗脱溶媒为80mL70%乙醇,洗脱速率为2mL·min-1;树脂经95%乙醇和1moL·L-1氢氧化钠溶液再生后,至少可重复使用5次。总黄酮(酚酸)与咖啡酸的保留率为75.56%及104.59%,峰1～5的保留率均达89%以上,纯化后总黄酮(酚酸)及咖啡酸的含量分别提高了3.99倍和4.69倍。结论 S-8型大孔吸附树脂能较好地纯化富集泽兰总黄酮(酚酸)。
Objective To study the technological conditions of the preparation of total flavonoids (phenolic acids) from Zeeland S-8 macroporous resin. Methods The total flavonoids (phenolic acids), caffeic acid content combined with the retention of the main characteristic peak area of the fingerprint were taken as indexes; the content of total flavonoids (phenolic acids) was determined by UV-Vis spectrophotometry; the content of caffeic acid was determined by HPLC; The main characteristic peak area. Results The optimum conditions for purifying total flavonoids (phenolic acids) of Zeeland were as follows: the concentration of sample solution was 0.1 g · mL-1 with crude drug, the sample loading was 10 g dry resin sample 7g crude drug, the adsorption rate was 1mL · min-1, the water eluting volume was 60mL, the eluting solvent was 80mL70% ethanol, the eluting rate was 2mL · min-1; the resin was oxidized by 95% ethanol and 1moL·L- After the sodium solution is regenerated, it can be reused for at least 5 times. The retention rates of total flavonoids (phenolic acids) and caffeic acid were 75.56% and 104.59%, respectively, and the retention rates of peak 1-5 were over 89%. The contents of total flavonoids and caffeic acid increased by 3.99 times And 4.69 times. Conclusion S-8 macroporous resin can better purify and enrich the total flavonoids of Zeeland (phenolic acids).