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分别在水杨酸和非水杨酸诱导下 ,研究了克新 1号和青薯 1 68脱毒苗在不同蔗糖浓度诱导培养基中试管微薯的发育状况 ,结果表明 :水杨酸影响微薯形成率 ,而蔗糖影响膨大率 ,二者协同决定微薯数量和重量。无水杨酸诱导时 ,8%～ 1 0 %糖浓度诱导效果最好 ,单瓶微薯数达 2 0枚左右 ,单薯平均重量 2 0 0 mg;0 .5mmol/L水杨酸诱导时 ,1 0 %～ 1 2 %蔗糖浓度效果最佳 ,单瓶微薯数达 30～ 40枚 ,单薯平均重量超过 1 0 0 mg,有效微薯的绝对数量最多。
Under the induction of salicylic acid and non-salicylic acid, the development of in vitro microtubers in vitro induced by different concentrations of sucrose was studied. The results showed that the effects of salicylic acid Potato formation rate, while the impact of sucrose swelling, the two jointly determine the number and weight of micro-potato. When salicylic acid was induced by no salicylic acid, the optimum concentration of 8% ~ 10% sugar was the best, and the number of single potato was about 20, the average weight of single potato was 200 mg. When 0.5 mmol / L salicylic acid was induced . The optimal concentration of sucrose was 10% ~ 12%. The number of single tubs was 30 ~ 40, the average weight of single potato was more than 100 mg, and the absolute amount of effective microtubes was the most.