论文部分内容阅读
目的建立寒凝血瘀大鼠体内羟基红花黄色素A(HSYA)的反相高效液相色谱分析方法,研究苏木对HSYA在寒凝血瘀模型大鼠体内药代动力学(药动学)的影响。方法寒凝血瘀模型大鼠12只,随机分为2组,分别灌服红花单煎液和红花-苏木合煎液,于给药后5、10、20、30、45、60、90、120、150、210、270min眼底静脉丛取血,20%三氯乙酸水溶液沉淀蛋白,采用RP-HPLC法检测给药不同时间后大鼠血浆中HSYA的浓度,DAS 2.0药动学软件计算药动学参数。结果与红花单用组相比,红花与苏木配伍后,大鼠血浆中HSYA的药时曲线下面积AUC(0-t)和峰浓度Cmax及分布半衰期t1/2α增大(P<0.01),表观分布容积V1/F和清除率CL/F减小(P<0.01)。结论苏木可促进寒凝血瘀模型大鼠对红花中HSYA的吸收,降低HSYA在体内的分布,从而发挥增效作用。
Objective To establish a reversed-phase high performance liquid chromatography (HPLC) method for the determination of hydroxysafflor yellow A (HSYA) in rats with cold-bloody stasis and to study the effect of hematoxylin-eosin (HSYA) on the pharmacokinetics influences. Methods 12 rats of model of cold coagulation and blood stasis were randomly divided into 2 groups. The decoctions of safflower and safflower decoction were drenched respectively. After 5, 10, 20, 30, 45, 60, 90, 120,150,210,270min retinal venous plexus blood, 20% aqueous solution of trichloroacetic acid precipitated protein, RP-HPLC method was used to detect rat plasma levels of HSYA after dosing, DAS 2.0 pharmacokinetic software to calculate the pharmacokinetic Learning parameters. Results Compared with the monocrotaline group, the AUC (0-t), the peak concentration Cmax and the half-life t1 / 2α of HSYA in the plasma of rats were significantly increased after compatibility of safflower and hematoxylin (P < 0.01). The apparent volume of distribution V1 / F and the clearance rate CL / F decreased (P <0.01). Conclusion Hematoxylin can promote the absorption of HSYA in safflowers in rats with cold and blood stasis syndrome, and reduce the distribution of HSYA in vivo and thus play a synergistic effect.