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目的探讨乙肝病毒前S1抗原和乙肝病毒DNA在乙肝病毒感染的不同阶段的动态诊断价值,阐述2者诊断差异性。方法采用ELISA法检测乙肝两对半,从检测结果中,随机筛选单纯HBsAg阳性标本50例,HBeAg阳性标本100例和Anti-HBs阳性标本100例,对3类标本采用ELISA法检测Pre-S1Ag和实时荧光定量PCR检测HBV-DNA。结果单纯HBsAg阳性标本中Pre-S1Ag阳性率为78%,HBV-DNA阳性率为12%,2者具有显著差异(P<0.01);HBeAg阳性标本Pre-S1Ag阳性率为84%,HBV-DNA阳性率为88%;Anti-HBs阳性标本中Pre-S1Ag阳性率为1%,HBV-DNA阳性率为28%,2者具有显著差异(P<0.01)。结论Pre-S1和HBV-DNA在HBV感染的动态诊断价值具有较大的差异性。在HBV感染早期Pre-S1优于HBV-DNA;在乙肝感染次早期2者具有高度一致性;乙肝感染恢复期2者具有较大的差异性。
Objective To investigate the dynamic diagnostic value of hepatitis B virus pre-S1 antigen and hepatitis B virus DNA in different stages of hepatitis B virus infection, and to explain the difference between the two. Methods Two-and-a-half hepatitis B was detected by ELISA. 50 samples of HBsAg-positive, 100 HBeAg-positive and 100 samples of Anti-HBs were randomly selected from the test results. Pre-S1Ag and Real-time fluorescence quantitative PCR detection of HBV-DNA. Results The positive rate of Pre-S1Ag in HBsAg-positive specimens was 78% and the positive rate of HBV-DNA was 12% (P <0.01). The positive rate of Pre-S1Ag in HBeAg-positive specimens was 84% The positive rate was 88%. The positive rate of Pre-S1Ag in positive anti-HBs was 1% and the positive rate of HBV-DNA was 28% (P <0.01). Conclusion The diagnostic value of Pre-S1 and HBV-DNA in HBV infection is quite different. Pre-S1 was better than HBV-DNA in the early stage of HBV infection, highly consistent in the second and second trimester of hepatitis B infection, and large difference in the recovery of hepatitis B infection.