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目的:提供一种简单可行,易于掌握的体外培养人晶体上皮细胞的方法。方法:用无齿显微镊子将晶体囊膜分离出来,剪碎后直接移至细胞培养瓶中培养,当细胞长出融合后,用胰蛋白酶消化传代。结果:接种培养4天后,可见晶体上皮细胞开始长出,并以贴壁方式生长。结论:用此方法体外培养人晶体上皮细胞,具有简单、快捷、成功率高的优点。眼科学报1997;13:170—172。
OBJECTIVE: To provide a simple and feasible method for culturing human lens epithelial cells in vitro. Methods: Toothless micro-tweezers were used to separate the capsular membrane. After shearing, the cells were directly transferred to the cell culture flask for culturing. When the cells grew confluent, they were passaged by trypsinization. RESULTS: Four days after inoculation, it was observed that the lens epithelial cells began to grow and grew in an adherent manner. Conclusion: Using this method to culture human lens epithelial cells in vitro has the advantages of simplicity, quickness and high success rate. Journal of Ophthalmology 1997; 13: 170-172.