目的寻求有针对性的抗疲劳措施，提高运动成绩。方法对２２名有体育特长男女大学生进行运动疲劳试验，分别在运动前、运动后立即及第２天取血，分离血清并测定乳酸脱氢酶（ＬＤＨ）及其同工酶、肌酸激酶（ＣＫ）及其同工酶和丙氨酸氨基转移酶（ＡＬＴ）活性。结果运动后血清酶活性均有明显升高，同工酶亦有变化，第２天逐渐恢复并接近正常水平。结论运动疲劳在一定程度上可引起组织细胞膜损伤，但这种损伤是可逆的，在短期内即可恢复 Objective To seek targeted anti-fatigue measures to improve athletic performance. Methods Twenty-two college male and female college students with physical education specialty were tested for exercise-induced fatigue. Blood samples were taken before exercise, immediately after exercise and on the second day after exercise. Serum lactate dehydrogenase (LDH) and its isozymes, creatine kinase CK) and its isozymes and alanine aminotransferase (ALT) activity. Results After exercise, serum enzyme activity were significantly increased, isoenzyme also changed, the first 2 days gradually recovered and close to normal levels. Conclusion Exercise fatigue can cause cell membrane damage to a certain extent, but this injury is reversible and can be recovered within a short period of time