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目的:探索通过高温环境、醋酸铅致敏和静脉注射脂多糖的综合方法制作大鼠温病暑热证模型的方法。方法:人工气候箱制造高温环境,麻醉动物,颈总动脉置入导管并连接生理多导仪,尾静脉注射醋酸铅致敏,1/2h后尾静脉注射脂多糖,动态观察肛温、脉搏、呼吸、收缩压、舒张压和脉压差,检测炎症介质IL-1β、IL-2、IL-4、IL-6、IL-10、IL-18、TNF-α,并进行肝、肺病理检查。结果:模型组的脉搏较对照组增快,肛温、呼吸、收缩压、舒张压和脉压差无组间差别;模型组的IL-1β、IL-4、IL-6、IL-10、TNF-α高于对照组,差别有统计学意义;模型组在4、5、6h的死亡率高于对照组,差别有统计学意义。结论:成功建立了大鼠温病暑热证模型,该模型病情比较严重,具有明显的炎症特点和中医暑热证的特点。
OBJECTIVE: To explore a method for making a warm rat model of Febrile Disease based on the comprehensive method of hyperthermia, sensitization with lead acetate and intravenous injection of lipopolysaccharide. Methods: The artificial climate chamber was used to make the high temperature environment. The animals were anesthetized, the common carotid artery was placed in a catheter and connected to a physiological polygraph. The lead was injected into the tail vein to sensitize the lead and the lipopolysaccharide was injected into the caudal vein after 1 / 2h. IL-1β, IL-2, IL-4, IL-6, IL-10, IL-18 and TNF-α in the inflammatory mediators were determined. Results: Compared with the control group, the pulse of the model group was faster than that of the control group. There was no significant difference in the rectal temperature, respiration, systolic pressure, diastolic pressure and pulse pressure between the two groups. The levels of IL-1β, IL-4, IL-6, IL- TNF-α higher than the control group, the difference was statistically significant; the mortality rate of the model group at 4,5,6 h was higher than that of the control group, the difference was statistically significant. Conclusion: The warm-heat rat syndrome model of rat warm-heart disease has been successfully established. The model is more serious with obvious inflammation characteristics and TCM syndrome.