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目的了解萧山区宋内志贺菌毒力基因分布和PFGE分子分型情况。方法采用PCR方法检测宋内志贺菌毒力基因并用脉冲场凝胶电泳(pulse-field gel electrophoresis,PFGE)进行分子分型和聚类分析。结果 130株宋内志贺菌中毒力基因set1A、set1B、sen、ipaH和ial的携带率分别为0%、0%、76.2%、100%和75.4%。用PFGE分型方法将130株菌株分成80个型别,相似性72.4%~97.9%,菌株数较多的有A、B、C 3个克隆群。结论本区宋内志贺菌毒力基因携带率高,具有较强致病性。PFGE分型结果说明2007年-2010年之间萧山的宋内志贺菌来源具有多样性,A、B、C 3个克隆群的菌株占据流行主导地位,应引起重视。
Objective To understand the virulence gene distribution and PFGE molecular typing of Shigella sonnensis in Xiaoshan district. Methods The virulence genes of Shigella sonnei were detected by PCR and the molecular typing and cluster analysis were performed by pulse-field gel electrophoresis (PFGE). Results The virulence genes of set1A, set1B, sen, ipaH and ial of 130 strains of Shigella sonnei were 0%, 0%, 76.2%, 100% and 75.4%, respectively. 130 isolates were divided into 80 genotypes by PFGE typing method and the similarities were 72.4% ~ 97.9%. There were 3 clonal groups A, B and C with more strains. Conclusion The virulence genes of Shigella sonnei in this area have high carriage rate and strong pathogenicity. The results of PFGE typing showed that the sources of Shigella sonnei in Xiaoshan from 2007 to 2010 were diversified. The strains of A, B and C clonal populations dominated the epidemic and should be taken seriously.