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目的观察持续游泳训练和间歇性负重游泳训练后大鼠杏仁核环磷酸腺苷(cAMP)浓度变化。方法雄性SD大鼠55只,随机分为对照组(NS)、持续训练组(CS)、间歇训练组(IS)。建立大鼠持续游泳和间歇性负重游泳训练模型,采用ELISA法,观察大鼠杏仁核cAMP浓度变化,并进行统计分析。结果与NS组相比,CS组大鼠游泳训练后即刻杏仁核cAMP浓度显著升高(P<0.05),至30 min时达到最高值(P<0.01),而60 min时则下降到最低值(P<0.01),120 min时又呈显著性升高(P<0.05),至240 min时又有所下降,再度低于NS组(P<0.05);间歇训练后,IS组大鼠杏仁核cAMP浓度即刻时较NS组显著降低(P<0.05),至30 min时达到最低值(P<0.01),而60 min时cAMP浓度值则达到最高值(P<0.01),之后120 min时又开始显著下降(P<0.01),但至240 min时呈升高的趋势,且再度高于NS组(P<0.05)。结论持续游泳训练与间歇性负重游泳训练后杏仁核cAMP浓度变化均呈反复升降的锯齿状特征,cAMP对不同形式运动性应激的反应具有较高灵敏度。
Objective To observe the changes of cAMP in amygdala after continuous swimming training and intermittent weight-bearing swimming training. Methods Fifty-five male SD rats were randomly divided into control group (NS), continuous training group (CS) and intermittent training group (IS). The rats sustained swimming and intermittent swimming load training model were established. The changes of cAMP concentration in amygdala of rats were observed by ELISA, and statistical analysis was made. Results Compared with NS group, the cAMP concentration of amygdala in CS group increased significantly (P <0.05), reached the highest level at 30 min (P <0.01), and dropped to the lowest at 60 min (P <0.01) and significantly increased at 120 min (P <0.05), and then decreased again at 240 min, again lower than that of NS group (P <0.05). After intermittent training, The concentration of cAMP in the nucleus decreased immediately (P <0.05) and reached its lowest level at 30 min (P <0.01), and reached the peak value at 60 min (P <0.01). At 120 min (P <0.01). However, it began to increase at 240 min and was again higher than that of NS group (P <0.05). Conclusions The cAMP concentration in amygdala shows a serrated pattern with repeated ascending and descending steps after continuous swimming training and intermittent weight-bearing swimming training. CAMP has high sensitivity to different forms of exercise-induced stress.