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目的：研究树突状细胞（ｄｅｎｄｒｉｔｉｃｃｅｌｓ，ＤＣ）类瘤苗免疫小鼠脾淋巴细胞对实验性骨髓瘤的继承性免疫预防与免疫治疗的作用。方法：先前用骨髓ＤＣ作为佐剂，研制了ＤＣ与肿瘤细胞的融合瘤苗，以及用灭活肿瘤细胞及其裂解产物体外脉冲的ＤＣ疫苗，并成功地诱导宿主产生抗肿瘤的免疫保护性反应，且对荷瘤动物具有免疫治疗作用。在此基础上，本研究用ＤＣ融合瘤苗及肿瘤抗原脉冲ＤＣ疫苗免疫的脾淋巴细胞作为免疫效应细胞，继承性转输到宿主体内，以观察其免疫预防与治疗的效果。结果：继承性免疫预防试验表明，转输免疫脾淋巴细胞后２组宿主均能够抵抗野生性肿瘤细胞的多次攻击，＞６０ｄ未见皮下移植性肿瘤形成，其６０ｄ存活率为１００％（５／５），与对照组相比，Ｐ＜０．０１。继承性免疫治疗（ａｄｏｐｔｉｖｅｉｍｍｕｎｏｔｈｅｒａｐｙ，ＡＩＴ）试验显示：①单用免疫淋巴细胞进行ＡＩＴ的２组，６０ｄ存活率分别为８０％（４／５）和６０％（３／５），其存活时间也明显延长，Ｐ＜０．０５；②先行化疗后进行ＡＩＴ，以及术前或术后再行ＡＩＴ，６０ｄ存活率提高到１００％（５／５），其存活时间显著延长，Ｐ＜０．０１；ＡＩＴ后宿主荷瘤生长明显被抑制，且部分荷瘤消退甚或治?
OBJECTIVE: To study the role of splenic lymphocytes from mice immunized with dendritic cels (DC) vaccines against the inherited immune prevention and immunotherapy of experimental myeloma. METHODS: Previously, bone marrow DCs were used as adjuvants to prepare DC-tumor cell fusion vaccines and DC vaccines that were pulsed in vitro with inactivated tumor cells and their lysates, and successfully induced the host to produce an anti-tumor immune protective response. , And have immunotherapy for tumor-bearing animals. On this basis, in this study, splenic lymphocytes immunized with DC-fused tumor vaccine and tumor antigen pulsed DC vaccine were used as immune effector cells, and they were inherited and transfused into the host to observe the effects of their immune prevention and treatment. Results: The inherited immunoprevention test showed that after transfusion of immune spleen lymphocytes, both groups of hosts were able to resist multiple attacks of wild tumor cells, and no subcutaneous transplantable tumors were formed after >60 days, and the 60-day survival rate was 100% (5 /5), P<0.01 compared with the control group. The inherited immunotherapy (AIT) test showed that: 1) Immune lymphocytes were used in 2 groups of AIT alone. The survival rate at 60 days was 80% (4/5) and 60% (3/5), respectively. The survival time was also significant. Prolonged, P <0.05; 2 AIT after preoperative chemotherapy, and preoperative or postoperative AIT, 60d survival rate increased to 100% (5/5), its survival time was significantly prolonged, P <0.01; After AIT the host tumor growth was significantly inhibited, and some tumors subsided or even treated?