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目的了解手足口病患儿感染EV71的情况,为科学防治疾病提供依据。方法采集手足口病患儿的咽拭子标本,接种于RD细胞,出现致细胞病变效应(CPE),运用RT-PCR方法,EV71引物检测样本和细胞培养物上清。结果标本直接用RT-PCR扩增,阳性率为30%(6/20),细胞培养后出现CPE的阳性率为45%(9/20),培养后经RT-PCR扩增阳性率为70%(14/20)。结论病毒分离和RT-PCR方法联合应用可以提高肠道病毒EV71的检出率,RT-PCR方法可以满足病毒流行期间同时处理大量样本的需要。
Objective To understand the situation of EV71 infection in children with hand-foot-mouth disease and provide scientific basis for prevention and treatment of disease. Methods Throat swab specimens from children with hand-foot-mouth disease were collected and inoculated into RD cells to induce cytopathic effect (CPE). RT-PCR and EV71 primers were used to detect the samples and cell culture supernatants. The positive rate of CPE was 30% (6/20). The positive rate of CPE after cell culture was 45% (9/20), and the positive rate of RT-PCR after culturing was 70 % (14/20). Conclusion The combined application of virus isolation and RT-PCR can improve the detection rate of enterovirus EV71, RT-PCR method can meet the needs of dealing with a large number of samples simultaneously during the virus epidemic.