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利用一种长光程薄层光谱电化学池，建立了在抗坏血酸存在下测定多巴胺的紫外-可见光谱电化学法。测定条件为：在pH=6．0的PBS介质中，电极电位在0．6V（vs.Ag/AgCl），测定波长为300nm多巴胺电氧化产物的吸光度，吸光度与多巴胺浓度在4×10－6～2．4×10－4mol/L范围内呈线性关系，检测限为1．0×10－6mol/L。用于多巴胺针剂的测定，结果令人满意。
Using a long pathlength thin-film spectroscopy electrochemical cell, an ultraviolet-visible spectroscopy electrochemical method for the determination of dopamine in the presence of ascorbic acid was established. The measurement conditions were as follows: the electrode potential was 0.6 V (vs. Ag / AgCl) in a PBS medium of pH 6.0, the absorbance of the dopamine electrooxidation product at a wavelength of 300 nm was measured, the absorbance and dopamine concentration were in the range of 4 × 10 -6 ~ 2.4 × 10-4mol / L within a linear range, the detection limit of 1.0 × 10-6mol / L. For the determination of dopamine injection, the results are satisfactory.