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目的研究胃癌组织Ｐ５３蛋白和增殖细胞核抗原（ＰＣＮＡ）的表达意义．方法应用ＡＢＣ免疫组织化学技术检测７６例保存资料完整并经病理学证实的胃癌石蜡包埋组织和３０例正常胃粘膜Ｐ５３蛋白及ＰＣＮＡ的表达进行同步检测．结果正常胃粘膜无Ｐ５３蛋白表达，而６４４％（４９／７６）胃癌组织中Ｐ５３蛋白表达阳性，Ｐ５３蛋白表达阳性者其细胞增殖活性为８２１％±１７６％，明显高于Ｐ５３蛋白表达阴性组的６４２％±１４３％（Ｐ＜００１）．Ｐ５３蛋白阳性率和ＰＣＮＡ计数值与胃癌临床分期及复发呈正相关（Ｐ＜００１）．结论联合检测Ｐ５３蛋白和ＰＣＮＡ对胃癌诊断、分期及预后有重要价值．
5. Objective To study the expression of P53 protein and proliferating cell nuclear antigen (PCNA) in gastric cancer. Methods ABC immunohistochemical technique was used to detect the expression of P53 protein and PCNA in gastric cancer tissues and 30 normal gastric mucosa specimens. Results There was no expression of P53 protein in normal gastric mucosa, but positive expression of P53 protein was found in 64.4% (49/76) gastric cancer tissues. The positive rate of P53 protein expression was 82.1%±17.6%, which was significantly higher than that of P53 protein. 64. 2% ± 14. 3% of P53 protein negative group (P <0. 01). The positive rate of P53 protein and the count of PCNA were positively correlated with clinical stage and recurrence of gastric cancer (P<001). 1. Conclusion Combined detection of P53 protein and PCNA has important value in the diagnosis, staging and prognosis of gastric cancer.