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各自主要分泌IFN γ和IL 4的Th1和Th2亚群 ,与临床疾病的关系十分密切。如何从表面标志上加以区分是一项迫切需要解决的问题。ST2是近年来提出的Th2细胞的稳定标志物。本工作在体外成功地诱导人脐带血T细胞向Th1或Th2分化的基础上 ,应用逆转录PCR分析了ST2mRNA的表达特点。证实ST2在人Th2细胞上的选择性表达。为了探索ST2、Th2与支气管哮喘的关系 ,本工作进一步检测了正常人和支气管哮喘患者外周血单个核细胞中 β actin、ST2以及IFN γ和IL 4的mRNA水平。结果显示 :支气管哮喘患者ST2mRNA水平升高 ,IL 4水平也明显升高 ,但IFN γ无变化。这提示ST2作为Th2细胞的标志物 ,有可能成为Th2极化性疾病如哮喘发病机制研究的一个参考性标志 ,至于ST2是否有可能作为治疗的靶分子 ,有待进一步探讨
The Th1 and Th2 sub-populations, each of which mainly secrete IFN [gamma] and IL4, are closely related to clinical disease. How to differentiate from the surface signs is an urgent problem to be solved. ST2 is a stable marker of Th2 cells that has been proposed in recent years. In this work, we successfully induced the differentiation of human umbilical cord blood T cells to Th1 or Th2 in vitro and analyzed the expression of ST2 mRNA by RT-PCR. Selective expression of ST2 on human Th2 cells was confirmed. In order to explore the relationship between ST2, Th2 and bronchial asthma, we further examined the mRNA levels of β actin, ST2, IFN γ and IL 4 in peripheral blood mononuclear cells of normal and bronchial asthma patients. The results showed that the level of ST2 mRNA in bronchial asthma patients was elevated, and the level of IL-4 was also elevated, but the level of IFN-γ was unchanged. This suggests that ST2 as a marker of Th2 cells may become a reference marker for the study of the pathogenesis of Th2 polarized diseases such as asthma. Whether ST2 may be used as a target for therapy remains to be further explored