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目的:建立HPLC同时测定桃儿七药材中苦鬼臼毒素葡萄糖二苷、山柰酚葡萄糖苷、4′-去甲鬼臼毒素、鬼臼毒素葡萄糖苷、槲皮素、鬼臼毒素、鬼臼毒酮、山柰酚含量的方法。方法:采用Hy PURITY C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.4%磷酸水溶液为流动相,进行梯度洗脱,流速0.8 m L·min~(-1),柱温25℃,检测波长290 nm。结果:苦鬼臼毒素葡萄糖二苷、山柰酚葡萄糖苷、4′-去甲鬼臼毒素、鬼臼毒素葡萄糖苷、槲皮素、鬼臼毒素、鬼臼毒酮、山柰酚的质量浓度分别在3.6~36、3.84~38.4、12.96~129.6、39.69~396.9、18.8~188、113.92~1132.9、5.6~56、14.4~144μg·m L~(-1)范围内与峰面积呈良好的线性关系;平均加样回收率(n=9)均高于98%,RSD小于3.0%;22批桃儿七样品中上述8个成分的含量范围分别为0.309~2.047、0.229~1.730、1.536~7.467、3.171~22.631、2.071~9.724、11.727~66.420、0.688~3.177、1.730~7.331 mg·g~(-1)。结论:本方法适用于桃儿七中有效成分的含量测定。
Objective: To establish an HPLC method for the simultaneous determination of picropodophyllin, glucoraphanin, 4’-detoxypodophyllotoxin, podophyllotoxin glucoside, quercetin, podophyllotoxin, Toxoid, kaempferol content method. Methods: The mobile phase consisted of HyPURITY C18 column (250 mm × 4.6 mm, 5 μm) with methanol-0.4% phosphoric acid as mobile phase at a flow rate of 0.8 m L · min -1 with a column temperature of 25 ℃ , Detection wavelength 290 nm. Results: The mass concentrations of picropodophyllin, glucoraphanin, 4’-demethoxypodophyllotoxin, podophyllotoxin glucoside, quercetin, podophyllotoxin, podophyllotoxin and kaempferol Respectively, in the range of 3.6 ~ 36,3.84 ~ 38.4,12.96 ~ 129.6,39.69 ~ 396.9,18.8 ~ 188,113.92 ~ 1132.9,5.6 ~ 56,14.4 ~ 144μg · m L ~ (-1) (N = 9) were higher than 98% and the RSD was less than 3.0%. The contents of the above eight components in 22 samples were 0.309 ~ 2.047, 0.229 ~ 1.730, 1.536 ~ 7.467 , 3.171 ~ 22.631,2.071 ~ 9.724,11.727 ~ 66.420,0.688 ~ 3.177,1.730 ~ 7.331 mg · g -1. Conclusion: The method is suitable for the determination of the active ingredients in Taoyuqi.