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采用抗白细胞介素2受体(IL-2R)的单克隆与多克隆抗体以及辣根过氧化物酶(HRP)标记的羊抗兔IgG,建立了高特异性及高灵敏度的双抗体夹心法测定可溶性白细胞介素2受体(sIL-2R)。结果,酶联免疫吸附试验(ELISA)测得兔抗人IL-2R多克隆抗体效价达1:10~4,免疫双向扩散法测得羊抗兔抗体效价高达1:256。本试剂盒检测sIL-2R的特异性强,灵敏度为100U/ml,在28C存放一年,稳定性不变。此方法操作简单,适用于临床和基础研究。
Using monoclonal and polyclonal antibodies against interleukin 2 receptor (IL-2R) and goat anti-rabbit IgG labeled with horseradish peroxidase (HRP), a high specificity and high sensitivity double antibody sandwich method Soluble interleukin 2 receptor (sIL-2R) was measured. As a result, the titer of rabbit anti-human IL-2R polyclonal antibody was 1:10 ~ 4 measured by enzyme-linked immunosorbent assay (ELISA), and the titer of goat anti-rabbit antibody was as high as 1: 256. This kit detects sIL-2R specificity, sensitivity of 100U / ml, stored at 28C for one year, the stability of the same. This method is simple, suitable for clinical and basic research.