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目的 观察低能量高功率密度毫米波照射对实验性肝癌大鼠演变过程的影响。方法 30只Wistar雄性大鼠分为 6组 ,第 1至 5组均为诱导肝癌组 ,用二乙基亚硝胺 (DEN ,diethylnitrosamine)喂养 14周诱导实验性肝癌 ,同时用毫米波通过直接或循经 (肝俞 )照射 5或 10周 ;第 6组为正常大鼠毫米波照射对照组。检测肝癌演变过程中血清学、组织学的变化。结果 诱导的肝癌大鼠 ,血清中γ 谷氨酰转肽酶 (γ GT)、碱性磷酸酶 (AKP)、胆汁酸 (BA)较正常大鼠组明显升高 ,毫米波照射肝癌大鼠γ GT、AKP、BA与肿瘤对照组相比略下降 ,统计学上各组间无明显差异。肿瘤对照组光镜下有肝癌结节 ,而毫米波照射组仅见嗜酸性和嗜碱性增生结节。毫米波照射后肝癌大鼠细胞核增殖数 (PCNA)明显低于肿瘤对照组 ,第 5周开始的直接毫米波照射组PCNA低于其余毫米波照射组 ;毫米波照射后肝癌大鼠 p5 3,bcl 2蛋白表达较肿瘤对照组明显减少。 结论 毫米波照射能减缓DEN对大鼠的毒性作用 ,肝脏病理改变减轻 ,能使DEN诱导肝肿瘤大鼠肝细胞增殖数降低及可能诱导部分变异的肝细胞凋亡
Objective To observe the effects of low energy and high power density millimeter wave irradiation on the evolution of experimental liver cancer rats. METHODS: Thirty male Wistar rats were divided into 6 groups. The first to fifth groups were all induced liver cancer. Experimental liver cancer was induced with diethylnitrosamine (DEN, diethylnitrosamine) for 14 weeks. Followed by the (liver) irradiation for 5 or 10 weeks; Group 6 is a normal rat millimeter wave irradiation control group. Detection of changes in serological and histological processes during the evolution of liver cancer. Results The serum levels of γ-glutamyl transpeptidase (γ GT), alkaline phosphatase (AKP) and bile acid (BA) in the induced liver cancer rats were significantly higher than those in normal rats. GT, AKP, and BA were slightly decreased compared with the tumor control group, and there was no statistically significant difference between the groups. The tumor control group had hepatocarcinoma nodules under light microscopy, while the millimeter-wave irradiation group had only eosinophilic and basophilic nodules. The PCNA of hepatocellular carcinoma cells after millimeter wave irradiation was significantly lower than that of the tumor control group. The PCNA of the direct millimeter wave irradiation group started at the 5th week was lower than that of the other millimeter wave irradiation groups; the p5 3 and bcl of the liver cancer rats after the millimeter wave irradiation. 2 Protein expression was significantly reduced compared with tumor control group. Conclusion Millimeter-wave irradiation can reduce the toxic effects of DEN on rats and reduce the pathological changes of the liver. It can reduce the hepatocyte proliferation of DEN-induced hepatic tumor rats and may induce partially mutated hepatocyte apoptosis.