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一个树种的群体交配系统(Matingsystem)提供了该树种是如何将其遗传物质从一个世代传递到下一个世代的基本信息。因此交配系统研究可有助于:(1)推测群体的基因型组成;(2)分析多基因位点连锁不平衡程度;(3)推测种子园的种子质量及提供种子园营建设计的有关信息;(4)在一定的程度上,可用于分析天然群体的遗传结构分化水平。在落叶松的10个种和3个变种中,目前已报道了2个种的交配系统:一个是美洲落叶松(L.laricina[DuRoi]K.Koch),另一是欧洲落叶松(L.deciduaMiler)。我国兴安、长白及华北落叶松的交配系统的研究尚未进行。本研究目的就是应用同功酶标记:(1)测定和比较这3个兴安落叶松种间及种内群体的异交率;(2)比较与其它针叶树的交配系统差异;(3)推测群体内自交或双亲近交发生的可能性。本文采用淀粉凝胶电泳技术,应用由6个酶系(PGI,MDH,6PGD,AAT,PGM及SDH)产生的7个多态性位点的同功酶标记分析了我国3个落叶松种(兴安、长白及华北落叶松)的共9个天然群体交配系统。结果发现兴安落叶松的加格达奇(内蒙古)群体表现出几乎完全的异交(tm=0.986±0.081)。华?
A species-based Mating system provides basic information about how the species pass on their genetic material from one generation to the next. Therefore, the study of mating system can help to: (1) infer the genotype composition of the population; (2) analyze the degree of linkage disequilibrium among multiple loci; (3) infer the seed quality of the seed orchard and provide relevant information ; (4) to a certain extent, can be used to analyze the genetic structure of the natural population differentiation level. In 10 species and 3 varieties of larch, two kinds of mating systems have been reported: one is L. laricina [DuRoi] K.Koch and the other is Larix. deciduaMiler). The research on the mating system of Xing’an, Changbai and Larix principis-rupprechtii in our country has not been carried out yet. The purpose of this study was to apply isozyme markers: (1) to determine and compare the outcrossing rates among three interspecific and intraspecific populations of Larix gmelinii; (2) to compare the differences in mating systems with other conifers; and (3) The possibility of inbreeding or inbreeding of parents. In this paper, 7 polymorphic loci produced by 6 enzymes (PGI, MDH, 6PGD, AAT, PGM and SDH) were analyzed by using starch gel electrophoresis. Three larch species Xing’an, Changbai, and Larix principis-rupprechtii) in a total of nine natural population mating systems. The results showed that the Jiagedaqi (Inner Mongolia) population of Larix gmelinii showed almost complete outcrossing (tm = 0.986 ± 0.081). China?