论文部分内容阅读
微小RNA(microRNA,miRNA)是来源于内源性发卡型转录本的单链非编码RNA,长度约22 nt,通过抑制或降解靶信使RNA(mRNA)的方式执行转录后基因调控作用。有研究表明,miRNA与生物的发育进程[1]、造血过程[2]、细胞生长分化与凋亡[3]、脂肪代谢[4]等生命过程有密切关系。miRNA的来源和特点Lee等[5]首次发现秀丽线虫幼虫的发育受lin-4基因调控,后者不编码蛋白质,而编码一对小RNA;Ambros和Ruvkun实验室随后发现lin-4可以与lin-14 mRNA 3’多个非翻译区(UTR)特异性结合,提出lin-4通过某种方式调节lin-14表达影响幼虫发育;随即Ruvkun实验室继续探索,揭
MicroRNAs (miRNAs) are single-stranded, non-coding RNAs derived from endogenous hairpin transcripts of approximately 22 nt in length and perform post-transcriptional gene regulation by inhibiting or degrading target messenger RNA (mRNA). Some studies have shown that miRNAs are closely related to biological processes such as developmental processes [1], hematopoiesis [2], cell growth and differentiation [3], and lipid metabolism [4]. The origin and characteristics of miRNA Lee et al [5] for the first time found that the development of C. elegans larvae is regulated by the lin-4 gene, which encodes a small RNA that does not encode a protein; Ambros and Ruvkun Laboratories subsequently found that lin-4 can interact with lin -14 mRNA specific for 3 ’UTRs, suggesting that lin-4 regulates lin-14 expression by some means affecting larval development; Rukkun Laboratories continues to explore and expose