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用PCR法检测166例乙型肝炎病毒感染者血清HBV-DNA。结果显示:HBsAg、HBeAg、抗-HBc阳性,HBsAg、抗-HBe、抗-HBc阳性和HBeAg阳性组,HBV-DNA阳性率分别为96.2％、28.6％、93.5％、HBsAg单项阳性者中,检出HBV-DNA阳性78例(孕妇占19.2％),阳性率81.3％。另外,在20例血清标志物全部阴性对照组中发现HBV-DNA阳性1例,阳性率5.36％。6例抗-HBc阳性和12例抗-HBe阳性者,HBV-DNA阳性率均为33.3％。从而提示乙肝病毒血清标志全部阴性,出现抗-HBs、抗-HBe、抗HBc,都不能说明血清中不存在乙肝病毒颗粒。因此应用PCR检测既可提高乙肝病毒感染的早期诊断率,又可直接反映病毒在体内复制状况。为指导临床治疗和预防提供可靠依据。
Serum HBV-DNA in 166 patients with hepatitis B virus infection was detected by PCR. The results showed that the positive rate of HBV-DNA of HBsAg, HBeAg, anti-HBc, HBsAg, anti-HBe, anti-HBc and HBeAg positive were 96.2%, 28.6%, 93.5% HBV-DNA positive 78 cases (19.2% of pregnant women), the positive rate of 81.3%. In addition, 20 cases of serum markers in all negative control group found HBV-DNA positive in 1 case, the positive rate of 5.36%. 6 cases of anti-HBc positive and 12 cases of anti-HBe positive, HBV-DNA positive rates were 33.3%. Thus suggesting that all hepatitis B virus serum markers were negative, anti-HBs, anti-HBe, anti-HBc, can not explain the absence of hepatitis B virus particles in serum. Therefore, the application of PCR detection can improve the early diagnosis of hepatitis B virus infection, but also directly reflect the virus in vivo replication status. To guide clinical treatment and prevention to provide a reliable basis.