论文部分内容阅读
[目的]构建Twist基因读码框架至真核表达载体pCDNA3.1,为进一步研究Twist蛋白的功能做材料准备。[方法]采用RT-PCR方法,以正常人子宫体组织cDNA为模板,扩增Twist基因全长读码框架,并构建到真核表达载体pCDNA3.1中。[结果]经过克隆测序结果证实,成功将Twist基因读码框架插入真核表达载体pCDNA3.1的相应位置;将pCDNA-Twist载体转染细胞系后,发现Twist蛋白在细胞中得到高水平的表达。[结论]Twist真核表达载体的成功构建,为深入研究Twist蛋白对肿瘤转移的作用提供了理想的材料。
[Objective] The purpose of this study was to construct the Twist gene reading frame to the eukaryotic expression vector pCDNA3.1 and prepare the material for further study on the function of Twist protein. [Method] RT-PCR method was used to amplify the full-length reading frame of Twist gene from cDNA of uterus tissue of normal people and constructed into eukaryotic expression vector pCDNA3.1. [Result] After cloned and sequenced, we successfully inserted the Twist gene into the corresponding site of the eukaryotic expression vector pCDNA3.1. After transfection of the pCDNA-Twist vector into the cell line, Twist protein was found to be highly expressed in the cells . [Conclusion] The successful construction of Twist eukaryotic expression vector provides an ideal material for further study of the effect of Twist protein on tumor metastasis.