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目的明确福建地区乙肝病毒(hepatitis B virus,HBV)基因型的分布情况及其特征。方法通过巢式PCR法分片段扩增慢性乙肝患者HBV全基因组序列,构建系统发育树确定福建地区乙型肝炎病毒主要基因型、血清型;同时结合HBV-DNA载量、血清标志物等检测结果分析福建地区乙肝基因型分布特征。结果从147例血清样本中成功扩增126例HBV完整基因序列;系统进化树分析表明,126例血清样本中B基因型78例(61.90%),C基因型42例(33.33%),B/C混合型6例(4.76%),未检测到其他基因型。血清型以adw2和adrq+为主,分别占63.49%和36.51%;不同基因型的分布与患者年龄、性别、HBV-DNA载量、所在地区均无相关性(P>0.05),而与患者血清标志物HBe Ag的阳性率、HBV血清型具有一定的相关性(P<0.05)。结论福建地区乙肝基因型主要以B型和C型为主,另见少量B/C混合型。B、C不同基因型的分布与患者血清标志物HBe Ag的阳性率及HBV的血清型具有一定相关性。
Objective To determine the distribution and characteristics of hepatitis B virus (HBV) genotypes in Fujian Province. Methods The whole genome sequence of hepatitis B virus was amplified by nested PCR and the major genotypes and serotypes of hepatitis B virus in Fujian area were constructed by phylogenetic tree. Combined with the detection results of HBV-DNA load and serum markers Analysis of Hepatitis B Genotype Distribution in Fujian Province. Results A total of 126 HBV genome sequences were successfully amplified from 147 serum samples. Phylogenetic tree analysis showed that B (78.9%), C (42.33%), B / C mixed type in 6 cases (4.76%), no other genotypes were detected. The serotypes were predominantly adw2 and adrq +, accounting for 63.49% and 36.51% respectively. The distribution of different genotypes had no correlation with patient’s age, gender, HBV-DNA load, and location (P> 0.05) The positive rate of marker HBe Ag was correlated with HBV serotype (P <0.05). Conclusion Hepatitis B genotypes in Fujian Province are mainly B type and C type, while a small amount of B / C mixed type is also seen. The distribution of different genotypes of B and C was correlated with the positive rate of serum HBeAg and the serotype of HBV.