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目的 研究CD40 抗原在恶性B淋巴细胞肿瘤中的表达及其CD40 抗原激发所致的生物学效应。方法 将激发型CD40 单克隆抗体 (单抗 ) 5C11加入肿瘤细胞的体外培养体系 ,采用细胞计数、流式细胞仪分析等方法分析单抗 5C11对不同肿瘤细胞株的作用。结果 5C11能引起CD40 表达强阳性的多发性骨髓瘤 (MM)细胞XG2发生同型聚集 ,抑制其生长并介导细胞凋亡 ;5C11可引起CD40+ 的恶性B淋巴瘤细胞株Daudi发生同型聚集 ,抑制其生长并使细胞发生G2 M期阻滞 ,但并不介导细胞凋亡。结论 激发型CD40 单抗通过诱导恶性B淋巴瘤细胞的凋亡或使G2 M期细胞阻滞 ,抑制肿瘤细胞的体外生长
Objective To study the expression of CD40 antigen in malignant B lymphocyte tumors and the biological effects of CD40 antigen challenge. Methods Activated CD40 monoclonal antibody (mAb) 5C11 was added to the in vitro culture system of tumor cells. Cell counting and flow cytometry analysis were used to analyze the effect of monoclonal antibody 5C11 on different tumor cell lines. Results 5C11 could induce XG2 homodimerization of multiple myeloma (MM) cells with strong CD40 expression, inhibit its growth and mediate apoptosis; 5C11 can cause homotypic aggregation of CD40+ malignant B lymphoma cell line Daudi, inhibiting it Grow and cause G2 M phase arrest in cells but do not mediate apoptosis. Conclusion The activated CD40 mAb inhibits the growth of malignant B lymphoma cells or blocks G2 M phase cells and inhibits the growth of tumor cells in vitro.