近年来,随着酶组织化学方法和超微结构细胞化学技术的不断进步,内耳酶组织细胞化学已发展成为一个十分活跃的研究领域。以酶组织化学方法和超微结构细胞化学技术研究内耳各部细胞的形态和功能,可由此获取组织内有关代谢的大量信息,这对于深入认识其病理生理过程的代谢基础,攻克一些关键性难题,以及推动耳科学和听力学前进都具有重要意义。兹就有关文献整理综述如下。【耳蜗螺旋器区域的酶组织细胞化学】一、碳酸酐酶(C AH):目前认为,CAH是参与内耳离子和液体主动调节的重要酶之一,同耳蜗电位的产生和组织呼吸有关。最近,Hsu等用Hansson氏法和改良Yokota氏法对豚鼠内耳CAH进行组织化学和细胞化学定位 In recent years, with the progress of enzymatic histochemical methods and ultrastructural cytochemistry, the enzyme in endolase cell cytochemistry has developed into a very active field of research. Enzymatic histochemistry and ultrastructural cytochemistry techniques can be used to study the morphology and function of various cells in the inner ear to obtain a large amount of information about the metabolism in the tissues. It is of great significance to understand the metabolic basis of the pathophysiological process and overcome some key problems. As well as to promote otology and audiology are of great significance. Here is a summary of the relevant literature as follows. 【Enzyme Cytochemistry in the Cochlear Spiral Zone】 I. Carbonic Anhydrase (C AH): At present, CAH is believed to be involved in the active regulation of ions and liquids in the inner ear. It is associated with the generation of cochlear potential and tissue respiration. Recently, Hsu et al. Used Hansson’s method and modified Yokota’s method to histochemically and cytochemically locate guinea pig’s inner ear CAH