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目的 :检测细胞免疫在血吸虫疫苗保护性免疫中的作用。方法 :用紫外线减毒日本血吸虫尾蚴 30 0± 5条免疫小鼠及日本血吸虫尾蚴 2 5± 3条感染小鼠。于第 2 wk、4 wk、8wk和 12 wk分别用血吸虫成虫抗原 ( SWAP)、虫卵抗原 ( SEA )及丝裂原 ( Con A或 L PS)体外刺激脾细胞和腹腔巨噬细胞 ( M) ,观察脾淋巴细胞的增殖反应及 M产生 IL- 1和脾细胞产生 IL- 2的活性的动态变化。结果 :两组鼠的脾细胞于免疫或感染后 2 wk- 8wk经 SWAP或 SEA刺激 T淋巴细胞增殖反应显著增强 ,第 12 wk呈现明显抑制 ;免疫组的 M和脾细胞经 SWAP或 SEA刺激于接种后第 4wk IL- 1和 IL- 2活性均显著增高 ,感染组的 M和脾细胞经 SWAP刺激 IL- 1于第 8wk- 12 wk活性增高、IL- 2于第 12 wk活性增高。结论 :提示减毒尾蚴免疫接种能较早地激活 T细胞增殖和细胞因子产生 ,在血吸虫保护性免疫中起重要作用。
PURPOSE: To examine the role of cellular immunity in protective immunity against schistosoma vaccine. Methods: The mice were immunized with 30 0 ± 5 immunized mice with attenuated Schistosoma japonicum cercariae and 25 ± 3 with Schistosoma japonicum cercariae. Splenocytes and peritoneal macrophages (M) were stimulated in vitro with SWAP, SEA and Con A or L PS at 2 wk, 4 wk, 8 wk and 12 wk, respectively ) To observe the proliferative response of splenic lymphocytes and the dynamic changes of the activity of IL-1 produced by M and IL-2 produced by spleen cells. Results: The proliferation of splenocytes in both groups was significantly increased after 2 weeks or 2 weeks of challenge with SWAP or SEA, and significantly inhibited at 12th week. M and splenocytes of the immunized group were stimulated by SWAP or SEA The activities of IL-1 and IL-2 were significantly increased at 4wk after inoculation. The activities of IL-1 in M and splenocytes of infected group were increased at 8th week-12th week and IL-2 at 12th week after SWAP stimulation. Conclusion: It is suggested that attenuated cercariae immunization can activate T cell proliferation and cytokine production earlier and play an important role in protective immunity of schistosomiasis.