脂蛋白-x(Lipoprotein-x,简称为Lp-x)的检测对临床上诊治肝胆疾病有越来越重要的价值,因而也被称为某些肝胆疾病的特异性指标。但目前国内检测Lp-x方法甚少且误差大。有鉴于此,我们建立起酶学方法检测Lp-x,经北京6家医院检验科使用认为酶法检测Lp-x有独特的优点。现总结如下。 反应原理。 患者血清含有大量的Lp,用适宜的沉淀剂将其除掉,Lp-x颗粒存在上清中,Lp-x含有定量的游离胆固醇(Fch),故可用胆固醇氧化酶 The detection of lipoprotein-x (Lp-x) is more and more important for the diagnosis and treatment of hepatobiliary diseases in clinic, and is also called the specific index of some hepatobiliary diseases. However, there are few ways to detect Lp-x in the country at present and the error is large. In view of this, we have established an enzymatic method for the detection of Lp-x. It has unique advantages of enzymatic detection of Lp-x by the use of six hospital laboratories in Beijing. Now summarized as follows. Reaction principle. The patient’s serum contains a large amount of Lp, which is removed by a suitable precipitating agent. Lp-x particles are present in the supernatant and Lp-x contains a fixed amount of free cholesterol (Fch). Therefore, cholesterol oxidase