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目的:研究白介素-10(IL-10)对实验性自身免疫性甲状腺炎小鼠的基因治疗作用。方法:将IL-10质粒DNA注射入由猪甲状腺球蛋白(pTg)诱发的自身免疫性甲状腺炎小鼠甲状腺内,pTg免疫后28天,进行甲状腺IL-10 mRNA表达和组织学等检查。结果:IL-10质粒DNA注射后1周或2周甲状腺均有IL-10 mRNA表达;转染IL-10质粒DNA的COS-7细胞48小时有显著IL-10 mRNA表达,同时转染后48、72小时细胞培养液中IL-10浓度显著增高;治疗组小鼠甲状腺淋巴细胞浸润指数(1.1±0.4)明显低于对照组(2.2±0.5)(P<0.01);治疗组小鼠血清IFN-Υ水平明显低于对照组(P<0.01)。结论:甲状腺直接注射编码IL-10质粒DNA能显著抑制自身免疫性甲状腺炎淋巴细胞对甲状腺的浸润,缓解病情的发展。
Objective: To investigate the gene therapy effect of interleukin-10 (IL-10) on experimental autoimmune thyroiditis in mice. Methods: IL-10 plasmid DNA was injected into the thyroid of mice with autoimmune thyroiditis induced by porcine thyroglobulin (pTg). The expression of IL-10 mRNA and histology were observed 28 days after pTg immunization. Results: IL-10 mRNA was expressed in the thyroid gland of IL-10 plasmid DNA 1 week or 2 weeks after injection. COS-7 cells transfected with IL-10 plasmid DNA had significant IL-10 mRNA expression 48 hours after transfection (P <0.01). The IL-10 concentration in the cell culture medium was significantly increased in 72 hours. The lymphocyte infiltration index (1.1 ± 0.4) in the treated group was significantly lower than that in the control group (2.2 ± 0.5) -Y levels were significantly lower than the control group (P <0.01). Conclusion: Direct injection of IL-10 plasmid DNA into the thyroid gland can significantly inhibit the infiltration of thyroid by lymphocytes of autoimmune thyroiditis and relieve the development of the disease.