【摘 要】
：
Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact
【机 构】
：
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beij
论文部分内容阅读
Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact that the hid-1 gene encodes a novel protein (HID-1) which is highly conserved from Caenorhabditis elegans to mammals, the domain structure, subcellular localization, and exact function of HID-1 remain unknown. Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain. In this study, we revealed that mammalian HID-1 localized to the medial-and transGolgi apparatus as well as the cytosol, and the localization was sensitive to brefeldin A treatment. Next, we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol. Finally, we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus. We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.
其他文献
Two mistakes in Fig.3A and 4A,respectively,were made due to typesetting errors.Figure 3A,on the left column of page 156,should be corrected as follows.
The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins.Successful me
Biologically important proteins related to membrane receptors,signal transduction,regulation,transcription,and translation are usually low in abundance and iden
Cholesterol-dependent cytolysins (CDC) are pore form-ing toxins. A prototype of the CDC family members is perfringolysin O (PFO), which directly binds to the ce
Estimating taxonomic content constitutes a key problem in metagenomic sequencing data analysis.However,extracting such content from high-throughput data of next
多功能电视墙系统包括三个主要部分——微机控制部分、电源及驱动电路、多屏组合显示部分,本文详细介绍微机控制电路的设计方法。N×N电视墙微机控制部分的主要任务是将完整
The human serum proteome is closely associated with the state of the body.Endogenous peptides derived from proteolytic enzymes cleaving on serum proteins are wi
以特征价格理论为基础,采用组合模型的形式建立了中山市商品住宅用地特征价格模型,并通过求偏导的方法测算了不同土地特征属性的边际土地增值量。结果表明:城市中心距、公共
１月９～１２日 学会秘书长封金芳出席北京大学成人教育学院在江苏省宜兴市召开的函授工作会议。会议主要议题：①布置函授站评估事宜；②布置１９９６年函授毕业生毕业工作；③商讨１９９６年招收函授生工