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目的:建立同种同基因和异基因大鼠子宫原位移植模型,评价移植子宫的生殖功能。方法:同基因组以雌性Wistar大鼠分别作为供体和受体,行子宫原位移植术。异基因组以雌性SD大鼠作供体,雌性Wistar大鼠作受体,行子宫原位移植术,受体大鼠术后第1天起使用环孢素,每天每公斤体重肌肉注射10mg。对照组雌性Wistar大鼠10只,切除左侧宫角。术后3组大鼠分别与同周龄雄性Wistar大鼠合笼12周,自然交配,观察受孕及分娩情况。子代大鼠自出生起每周称量体重至第8周,绘生长曲线,并各自分别交配。结果:10例同基因大鼠,10例异基因大鼠分别行子宫原位移植手术,10例对照手术。受体大鼠存活率同基因组80%(8/10),异基因组90%(9/10),对照组大鼠存活率90%(9/10)。同基因组8例存活的大鼠中4例受孕,受孕率50%,顺产分娩子代大鼠15只;异基因组9例存活大鼠中2例受孕,受孕率22.2%,但2例均胎死宫内;对照组9例存活大鼠中6例受孕,受孕率66.7%,顺产分娩子代大鼠19只。同基因组和对照组分娩的子代大鼠外观均无畸形,两组子代生长曲线基本相似,并有正常生殖功能。结论:同基因大鼠子宫原位移植后移植子宫可恢复正常生殖功能;异基因大鼠子宫原位移植后应用环孢素可使移植子宫长期存活,但生殖功能可能受影响。
OBJECTIVE: To establish a orthotopic transplantation model of allogenic allogeneic and allogenic rat to evaluate the reproductive function of the uterus. Methods: Isogenic female Wistar rats were used as donors and recipients, respectively, for uterine orthotopic transplantation. Allogeneic female Sprague-Dawley rats were used as donors and female Wistar rats as recipients. Uterine orthotopic transplantation was performed in allogeneic rats. Cyclosporine was given on the first day after operation in recipient rats, and intramuscular injection of 10 mg / kg body weight per day. The control group of female Wistar rats 10, the left side of the uterine horn. After operation, the rats in the three groups were respectively caged with male Wistar rats of the same age for 12 weeks and mated naturally to observe the pregnancy and childbirth. Offspring rats were weighed weekly until the 8th week after birth, and the growth curves were drawn and mated separately. Results: 10 cases of isogenic rats and 10 cases of allogeneic rats underwent uterine orthotopic transplantation and 10 cases of control operation. The survival rate of the recipient rats was 80% (8/10) in the genome, 90% (9/10) in the allogeneic group, and 90% (9/10) in the control group. In the same group of 8 surviving rats, 4 cases were conceptioned with a conception rate of 50% and 15 offspring were delivered by birth; 2 of 9 allogeneic survived rats were conceptioned with a conception rate of 22.2% Intrauterine; control group of 9 surviving rats in 6 cases of pregnancy, pregnancy rate was 66.7%, 19 offspring of childbirth delivery. There was no deformity in the appearances of the offsprings of the same genomic group and the control group. The growth curves of the offspring in the two groups were similar and had normal reproductive function. CONCLUSION: Normal uterine allografts can restore normal reproductive function after uterine orthotopic transplantation in vivo. Cyclosporine can improve long-term survival of uterine allografts in uterine allogenic rats, but reproductive function may be affected.