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目的筛选食管癌中差异表达的基因,进一步了解食管癌发生的分子机制。方法利用荧光差异显示技术(DD-PCR)从食管癌组织相对癌旁组织中获得差异表达片段,对这些片段进行克隆和序列分析。通过在GenBank中同源性检索,查找差异片段相对应的同源基因。利用定量PCR检测验证该基因表达的差异性。设计引物获得该基因全长。结果通过DD-PCR得到3个差异片段,分别为人类10号常染色体上的开放阅读框99;人类信号转导和激活转录因子2及人类omega蛋白(Igll3)。定量PCR验证的结果表明,Igll3在食管癌组织中的表达量高于癌旁组织。设计引物得到该基因全长,测序表明该基因长为711bp,编码236aa。结论 Igll3在食管癌组织中异常表达,可能在食管癌发生过程中起着重要的作用。
Objective To screen differentially expressed genes in esophageal cancer to further understand the molecular mechanism of esophageal cancer. Methods The differentially expressed fragments of esophageal cancer tissues were obtained by DD-PCR. The fragments were cloned and sequenced. Through homology search in GenBank, the corresponding homologous genes of different fragments were searched. The difference of the gene expression was verified by quantitative PCR. Primers were designed to obtain the full length of this gene. Results Three different fragments were obtained by DD-PCR. They were open reading frame 99 on human autosome 10; human signal transduction and activation of transcription factor 2 and human omega protein (Igll3). The results of quantitative PCR showed that the expression of Igll3 in esophageal cancer tissues was higher than that in paracancerous tissues. The full-length of this gene was designed by primers. Sequencing showed that the gene was 711 bp in length and encoded 236aa. Conclusion The abnormal expression of Igll3 in esophageal cancer tissues may play an important role in the carcinogenesis of esophageal cancer.