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目的 研究和探讨敏感期内及敏感期末不同时限单眼斜视（ ｍｏｎｏｃｕｌａｒ ｓｔｒａｂｉｓｍｕｓ， ＭＳ） 和剥夺（ｍｏｎｏｃｕｌａｒ ｄｅｐｒｉｖａｔｉｏｎ ， ＭＤ） 动物模型视觉系统神经营养因子－ ３ 塑性变化的规律， 可望能为临床寻找有效预防和治疗斜视和剥夺性弱视提供参考依据。方法 本实验应用免疫细胞化学ＳＡＢＣ 技术染色和计算机图像分析了神经营养因子－ ３ （Ｎｅｕｒｏｔｒｏｐｈｉｎ － ３ ， ＮＴ－ ３） 在正常、敏感期内及敏感期末单眼斜视和剥夺猫视皮质１７ 区、外侧膝状体神经元的表达。结果 在视皮质１７ 区， ＮＴ－ ３ 分布在２～６ 层， ＮＴ－ ３ 免疫阳性神经元核浆淡染呈浅棕褐色， 在锥体细胞也可见其突触近端， ＩＶ 层的免疫阳性细胞较多。在外侧膝状体免疫阳性神经元主要见于大细胞， 也有部分小细胞着色。结果显示ＮＴ－ ３ 免疫阳性表达浓度及数密度为， 在同侧视皮质１７ 区ＩＶ 层， 正常组与敏感期内单眼斜视和剥夺组比较具有显著性差异（ Ｐ＜ ０－０１） ， 正常组与敏感期末单眼斜视和剥夺组比较无显著性差异（ Ｐ＞０－０５） 。在同侧外侧膝状体正常眼输入层（Ａ１） 与敏感期内斜视和剥夺眼输入层（Ａ１） 比较有显著性差异（ Ｐ＜ ０－０５ ?
Objective To study and investigate the regularity of plasticity of neurotrophin - 3 in visual system of monocular strabismus (MS) and monocular deprivation (MD) animal model in sensitive period and at the sensitive end of period, and it is expected to find an effective method for clinical prevention And treatment of strabismus and deprivation amblyopia provide a reference. Methods Immunocytochemical SABC staining and computerized image analysis of neurotrophin - 3 (NT - 3) in monocular strabismus and deprivation of cat visual cortex in the normal, sensitive period and the sensitive period of 17 areas, the lateral knee The expression of neurons in the body. Results In the visual cortex, NT-3 was distributed on the second to sixth floor. The light nuclear staining of NT-3 immunoreactive neurons was pale brown, and the synapses were also seen in the pyramidal cells. The immunoreactivity of the IV layer was positive More cells. In the lateral geniculate immunoreactive neurons are mainly seen in large cells, but also some small cell coloring. The results showed that NT-3 immunoreactive expression density and number density in the ipsilateral visual cortex 17 area IV, normal and sensitive period monocular strabismus and deprivation group compared with significant difference (P <0-01), normal group There was no significant difference between monocular strabismus and deprivation group (P> 0-05). In the ipsilateral lateral geniculate body normal eye input layer (A1) and sensitive period of strabismus and deprived eye input layer (A1) were significantly different (P <0-05?