论文部分内容阅读
目的采用超高效液相色谱-串联四极杆质谱仪(UPLC-MS/MS),在电喷雾离子源正离子(ESI+)和多反应监测(MRM)模式下建立了定性、定量测定海洛因中10种掺杂物的方法。方法使用甲醇超声溶样,ACQUITY UPLCTMBEH C18(1.7μm,100 mm×2.1 mm)色谱柱,流动相为pH=9.66的10 mmol/L的碳酸氢铵和乙腈,在梯度条件下分析。结果该方法定量限为0.005-0.5μg/g。添加水平在25-250 ng/mL范围内,加标回收率为95.8%-114.2%,相对标准偏差为1.1%-6.5%。结论本方法简便、快速、准确、灵敏,满足对海洛因样品中掺杂物进行快速定性、定量分析的需要。
OBJECTIVE To establish a qualitative and quantitative method for determination of 10 heroin in the positive ion electrospray ionization (ESI +) and multi-reaction monitoring (MRM) modes using ultra performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-MS / MS) Species of dopants method. Methods The samples were separated on a ACQUITY UPLC TMBEH C18 (1.7 μm, 100 mm × 2.1 mm) column using methanol sonication and the mobile phase consisted of 10 mmol / L ammonium bicarbonate and acetonitrile at pH 9.66. Results The limit of quantification was 0.005-0.5 μg / g. The recoveries ranged from 95.8% -114.2% with relative standard deviations of 1.1% -6.5% at 25-250 ng / mL. Conclusion The method is simple, rapid, accurate and sensitive to meet the needs of rapid qualitative and quantitative analysis of dopants in heroin samples.