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目的:研究岩白菜素预处理对脂多糖(lipopolysaccharide,LPS)诱导RAW264.7细胞IL-6、TNF-α及NF-κB表达的影响。方法:将RAW264.7细胞随机分为空白对照组、DMSO组(DMSO浓度≤0.1%)、模型组(加入终浓度为10μg/mL的LPS)及岩白菜素低、中、高剂量组(0.5、5、50μmol/L),各组处理完毕后,ELISA检测不同处理组对LPS诱导RAW264.7细胞分泌白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量,Western blot方法检测岩白菜素对LPS诱导细胞核因子-κB(NF-κB)p65蛋白表达。结果:LPS能够明显诱导小鼠RAW264.7细胞IL-6、TNF-α产生,岩白菜素3个浓度均能抑制LPS诱导的IL-6、TNF-α产生,呈现剂量依赖性;小鼠RAW264.7细胞株产生的NF-κB p65表达可以被LPS诱导显著增加,岩白菜素3个浓度均能抑制LPS诱导产生的NF-κB p65表达。结论:岩白菜素预处理通过抑制NF-κB活化,进而减少炎性细胞因子IL-6、TNF-α的生成发挥抗炎作用。
Objective: To investigate the effect of bergein preconditioning on the expression of IL-6, TNF-α and NF-κB in RAW264.7 cells induced by lipopolysaccharide (LPS). Methods: RAW264.7 cells were randomly divided into blank control group, DMSO group (DMSO concentration ≤0.1%), model group (LPS plus 10μg / mL final concentration) and bergenin low, medium and high dose group , 5,50μmol / L). After treatment, the levels of IL-6 and TNF-α secreted by LPS-induced RAW264.7 cells in different treatment groups were detected by ELISA. Western blot was used to detect the effect of bergenin on the expression of nuclear factor-kappa B (NF-κB) p65 in LPS. Results: LPS could obviously induce the production of IL-6 and TNF-α in mouse RAW264.7 cells. Bergenin could inhibit the production of IL-6 and TNF-α induced by LPS in a dose-dependent manner. Mouse RAW264 .7 The expression of NF-κB p65 induced by LPS could be significantly increased by LPS induction, and all of Bergenin could inhibit the expression of NF-κB p65 induced by LPS. Conclusion: Bergenin pretreatment can inhibit the activation of NF-κB, and then reduce the production of inflammatory cytokines IL-6 and TNF-α to exert anti-inflammatory effects.