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实验以SD大鼠为研究对象,建立游泳训练模型,测试不同负荷下红细胞膜抗氧化酶(SOD)、ATP酶的活性及膜脂质过氧化产物丙二醛(MDA)含量的变化,旨在研究不同负荷游泳训练对大鼠红细胞膜脂质过氧化水平和ATP酶活性的影响。结果显示,低负荷运动训练可降低安静时红细胞膜上MDA含量,提高其抗氧化酶SOD活性,高负荷的训练则产生相反的结果;力竭运动后低负荷训练组红细胞膜Na~+,K~+-ATP酶活性增加,高负荷训练组该酶的活性下降;低负荷运动训练对Ca~(2+)-ATP酶活性无明显影响,高负荷训练组Ca~(2+)-ATP酶活性的下降。结论:适宜的运动训练可以增强红细胞膜的抗氧化能力,延长大鼠的力竭性时间。
The experiment was conducted in SD rats. The swimming training model was established to test the changes of activities of antioxidant enzymes (SODs) and ATPases and the contents of malondialdehyde (MDA) in membrane of erythrocytes under different loads. To study the effect of different load swimming training on lipid peroxidation and ATPase activity in rat erythrocyte membrane. The results showed that low exercise training could reduce the content of MDA in the erythrocyte membrane at rest and increase the activity of antioxidant enzyme SOD, and the training with high load produced the opposite result. After exhaustive exercise, the erythrocyte membrane Na ~ +, K The activity of ~ (+) - ATPase increased while the activity of Ca ~ (2 +) - ATPase was decreased in high exercise training group Activity decline. Conclusion: Appropriate exercise training can enhance the antioxidant capacity of erythrocyte membrane and prolong the exhaustion time in rats.