AIM: To investigate the chemical constituents of Selaginella sinensis(Desv.) Spring. METHODS: Chromatographic separations on Diaion HP-20, silica gel, and Sephadex LH-20 were used. The structures of the isolates were elucidated on the basis of spectroscopic analysis, as well as chemical methods. RESULTS: Eight compounds were obtained and their structures were identified as sinensioside A(1), syringaresinol-4-O-β-D-glucopyranoside(2),(+)-medioresinol-4-O-β-D-glucopyranoside(3), pinoresinol-4, 4′-di-O-β-D-glucopyranoside(4), quercetin(5), eucomic acid(6), shikimic acid(7), and 2, 3-dihydroamentoflavone(8). CONCLUSION: Compound 1 is a new dihydrobenzofuran sesquilignan glycoside from Selaginella sinensis. A: To investigate the chemical constituents of Selaginella sinensis (Desv.) Spring. METHODS: Chromatographic separations on Diaion HP-20, silica gel, and Sephadex LH-20 were used. The structures of the isolates were elucidated on the basis of spectroscopic analysis RESULTS: Eight compounds were obtained and their structures were identified as sinensioside A (1), syringaresinol-4-O-β-D-glucopyranoside (2) β-D-glucopyranoside (3), pinoresinol-4, 4’-di-O-β-D-glucopyranoside (4), quercetin (5), eucomic acid (6), shikimic acid -dihydroamentoflavone (8). CONCLUSION: Compound 1 is a new dihydrobenzofuran sesquilignan glycoside from Selaginella sinensis.