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目的:研究前列腺素Al(PGAl)对心脏微血管内皮细胞凋亡的影响.方法:培养分离大鼠心脏微血管内皮细胞,建立缺氧再给氧模型,通过原位缺口末端标记观察PGAl对内皮细胞凋亡的作用;通过凝胶电泳迁移率测定NF-κB的活性;用Western blot法测定Bcl-2和Bax蛋白表达;用Northern blot法测定bcl-2 mRNA的表达.结果:PGAl能明显减少缺氧再给氧内皮细胞的凋亡,抑制NF-κB的活性,升高Bcl-2蛋白及bcl-2 mRNA的表达,不改变Bax蛋白的表达,导致Bcl-2/Bax增加.结论:PGAl通过NF-κB介导抑制大鼠心脏微血管内皮细胞的凋亡.
Aims: To study the effect of PGAl on apoptosis of cardiac microvascular endothelial cells.Methods: Cultured rat cardiac microvascular endothelial cells (HUVECs) were cultured and hypoxia-reoxygenation model was established. Endothelial cell apoptosis was observed by in situ nick end labeling The activity of NF-κB was determined by the gel electrophoretic mobility shift assay. The expressions of Bcl-2 and Bax protein were determined by Western blot and the expression of bcl-2 mRNA by Northern blot.Results: PGAl could significantly reduce the hypoxia Bcl-2 / Bax was increased after apoptosis of the endothelial cells, and then the apoptosis of endothelial cells was inhibited, the activity of NF-κB was inhibited, the expression of Bcl-2 protein and bcl-2 mRNA was increased, the expression of Bax protein was not changed, κB Mediated Inhibition of Apoptosis in Rat Cardiac Microvascular Endothelial Cells.